A new protein expressed in bone marrow cells and osteoblasts with implication in osteoblast recruitment.
Identifieur interne : 003452 ( Main/Exploration ); précédent : 003451; suivant : 003453A new protein expressed in bone marrow cells and osteoblasts with implication in osteoblast recruitment.
Auteurs : T. Wurtz [Suède] ; A. Krüger ; C. Christersson ; C. LundmarkSource :
- Experimental cell research [ 0014-4827 ] ; 2001.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Analyse de séquence d'ARN, Animaux, Banque de gènes, Cellules de la moelle osseuse (métabolisme), Données de séquences moléculaires, Fémur (croissance et développement), Fémur (métabolisme), Hybridation in situ, Immunohistochimie, Ostéoblastes (), Ostéoblastes (métabolisme), Ostéogenèse, Protéines, Protéines du sang (), Protéines du sang (génétique), Protéines du sang (métabolisme), Rat Sprague-Dawley, Rats, Séquence d'acides aminés, Technique de Northern, Technique de Western.
- MESH :
- croissance et développement : Fémur.
- génétique : ARN messager, Protéines du sang.
- métabolisme : ARN messager, Cellules de la moelle osseuse, Fémur, Ostéoblastes, Protéines du sang.
- Analyse de séquence d'ARN, Animaux, Banque de gènes, Données de séquences moléculaires, Hybridation in situ, Immunohistochimie, Ostéoblastes, Ostéogenèse, Protéines, Protéines du sang, Rat Sprague-Dawley, Rats, Séquence d'acides aminés, Technique de Northern, Technique de Western.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Blood Proteins (chemistry), Blood Proteins (genetics), Blood Proteins (metabolism), Blotting, Northern, Blotting, Western, Bone Marrow Cells (metabolism), Femur (growth & development), Femur (metabolism), Gene Library, Immunohistochemistry, In Situ Hybridization, Molecular Sequence Data, Osteoblasts (chemistry), Osteoblasts (metabolism), Osteogenesis, Proteins, RNA, Messenger (genetics), RNA, Messenger (metabolism), Rats, Rats, Sprague-Dawley, Sequence Analysis, RNA.
- MESH :
- chemical , chemistry : Blood Proteins.
- chemical , genetics : Blood Proteins, RNA, Messenger.
- chemical , metabolism : Blood Proteins, RNA, Messenger.
- chemistry : Osteoblasts.
- growth & development : Femur.
- metabolism : Bone Marrow Cells, Femur, Osteoblasts.
- Amino Acid Sequence, Animals, Blotting, Northern, Blotting, Western, Gene Library, Immunohistochemistry, In Situ Hybridization, Molecular Sequence Data, Osteogenesis, Proteins, Rats, Rats, Sprague-Dawley, Sequence Analysis, RNA.
Abstract
To study osteoblast recruitment from bone marrow cells, a rat femur cDNA library was screened by in situ hybridization for novel mRNA sequences that are frequently expressed in both marrow cells and osteoblasts. One isolated clone, called RP59, is described here. Northern blots indicated two bands of 2.6 and 2.8 kb in femur and spleen, tissues containing high amounts of immature mesenchymal cells, and no or little expression in other tissues. The cDNA sequence revealed a reading frame for a repetitive protein composed of arrays of 14-mers and phased phosphorylation sites. Antisera versus RP59 detected a single band of 90 kDa by Western blotting of femur extract. Immunohistochemistry indicated strong RP59 presence in the cytoplasm of bone marrow cells and weaker presence in nuclei of osteoblasts. Intermediate stages were found between strongly labeled, round, free bone marrow cells and weaker labeled, fibroblast-like young osteoblasts associated with bone matrix. These data indicated that marrow cells with high RP59 content were recruited into growing bone tissue. RP59 may help to study the transition of bone marrow cell to osteoblast in more detail.
DOI: 10.1006/excr.2000.5114
PubMed: 11161722
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002566
- to stream PubMed, to step Curation: 002566
- to stream PubMed, to step Checkpoint: 002427
- to stream Ncbi, to step Merge: 000079
- to stream Ncbi, to step Curation: 000079
- to stream Ncbi, to step Checkpoint: 000079
- to stream Main, to step Merge: 003490
- to stream Main, to step Curation: 003452
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A new protein expressed in bone marrow cells and osteoblasts with implication in osteoblast recruitment.</title><author><name sortKey="Wurtz, T" sort="Wurtz, T" uniqKey="Wurtz T" first="T" last="Wurtz">T. Wurtz</name><affiliation wicri:level="1"><nlm:affiliation>Center for Oral Biology, Huddinge, 14104, Sweden. Tilmann.Wurtz@cob.ki.se</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Center for Oral Biology, Huddinge, 14104</wicri:regionArea><wicri:noRegion>14104</wicri:noRegion></affiliation></author><author><name sortKey="Kruger, A" sort="Kruger, A" uniqKey="Kruger A" first="A" last="Krüger">A. Krüger</name></author><author><name sortKey="Christersson, C" sort="Christersson, C" uniqKey="Christersson C" first="C" last="Christersson">C. Christersson</name></author><author><name sortKey="Lundmark, C" sort="Lundmark, C" uniqKey="Lundmark C" first="C" last="Lundmark">C. Lundmark</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11161722</idno><idno type="pmid">11161722</idno><idno type="doi">10.1006/excr.2000.5114</idno><idno type="wicri:Area/PubMed/Corpus">002566</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002566</idno><idno type="wicri:Area/PubMed/Curation">002566</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002566</idno><idno type="wicri:Area/PubMed/Checkpoint">002427</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002427</idno><idno type="wicri:Area/Ncbi/Merge">000079</idno><idno type="wicri:Area/Ncbi/Curation">000079</idno><idno type="wicri:Area/Ncbi/Checkpoint">000079</idno><idno type="wicri:doubleKey">0014-4827:2001:Wurtz T:a:new:protein</idno><idno type="wicri:Area/Main/Merge">003490</idno><idno type="wicri:Area/Main/Curation">003452</idno><idno type="wicri:Area/Main/Exploration">003452</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A new protein expressed in bone marrow cells and osteoblasts with implication in osteoblast recruitment.</title><author><name sortKey="Wurtz, T" sort="Wurtz, T" uniqKey="Wurtz T" first="T" last="Wurtz">T. Wurtz</name><affiliation wicri:level="1"><nlm:affiliation>Center for Oral Biology, Huddinge, 14104, Sweden. Tilmann.Wurtz@cob.ki.se</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Center for Oral Biology, Huddinge, 14104</wicri:regionArea><wicri:noRegion>14104</wicri:noRegion></affiliation></author><author><name sortKey="Kruger, A" sort="Kruger, A" uniqKey="Kruger A" first="A" last="Krüger">A. Krüger</name></author><author><name sortKey="Christersson, C" sort="Christersson, C" uniqKey="Christersson C" first="C" last="Christersson">C. Christersson</name></author><author><name sortKey="Lundmark, C" sort="Lundmark, C" uniqKey="Lundmark C" first="C" last="Lundmark">C. Lundmark</name></author></analytic><series><title level="j">Experimental cell research</title><idno type="ISSN">0014-4827</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blood Proteins (chemistry)</term><term>Blood Proteins (genetics)</term><term>Blood Proteins (metabolism)</term><term>Blotting, Northern</term><term>Blotting, Western</term><term>Bone Marrow Cells (metabolism)</term><term>Femur (growth & development)</term><term>Femur (metabolism)</term><term>Gene Library</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Molecular Sequence Data</term><term>Osteoblasts (chemistry)</term><term>Osteoblasts (metabolism)</term><term>Osteogenesis</term><term>Proteins</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sequence Analysis, RNA</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Analyse de séquence d'ARN</term><term>Animaux</term><term>Banque de gènes</term><term>Cellules de la moelle osseuse (métabolisme)</term><term>Données de séquences moléculaires</term><term>Fémur (croissance et développement)</term><term>Fémur (métabolisme)</term><term>Hybridation in situ</term><term>Immunohistochimie</term><term>Ostéoblastes ()</term><term>Ostéoblastes (métabolisme)</term><term>Ostéogenèse</term><term>Protéines</term><term>Protéines du sang ()</term><term>Protéines du sang (génétique)</term><term>Protéines du sang (métabolisme)</term><term>Rat Sprague-Dawley</term><term>Rats</term><term>Séquence d'acides aminés</term><term>Technique de Northern</term><term>Technique de Western</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Blood Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Blood Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Blood Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Osteoblasts</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Fémur</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Femur</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Protéines du sang</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bone Marrow Cells</term><term>Femur</term><term>Osteoblasts</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Cellules de la moelle osseuse</term><term>Fémur</term><term>Ostéoblastes</term><term>Protéines du sang</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blotting, Northern</term><term>Blotting, Western</term><term>Gene Library</term><term>Immunohistochemistry</term><term>In Situ Hybridization</term><term>Molecular Sequence Data</term><term>Osteogenesis</term><term>Proteins</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sequence Analysis, RNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ARN</term><term>Animaux</term><term>Banque de gènes</term><term>Données de séquences moléculaires</term><term>Hybridation in situ</term><term>Immunohistochimie</term><term>Ostéoblastes</term><term>Ostéogenèse</term><term>Protéines</term><term>Protéines du sang</term><term>Rat Sprague-Dawley</term><term>Rats</term><term>Séquence d'acides aminés</term><term>Technique de Northern</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To study osteoblast recruitment from bone marrow cells, a rat femur cDNA library was screened by in situ hybridization for novel mRNA sequences that are frequently expressed in both marrow cells and osteoblasts. One isolated clone, called RP59, is described here. Northern blots indicated two bands of 2.6 and 2.8 kb in femur and spleen, tissues containing high amounts of immature mesenchymal cells, and no or little expression in other tissues. The cDNA sequence revealed a reading frame for a repetitive protein composed of arrays of 14-mers and phased phosphorylation sites. Antisera versus RP59 detected a single band of 90 kDa by Western blotting of femur extract. Immunohistochemistry indicated strong RP59 presence in the cytoplasm of bone marrow cells and weaker presence in nuclei of osteoblasts. Intermediate stages were found between strongly labeled, round, free bone marrow cells and weaker labeled, fibroblast-like young osteoblasts associated with bone matrix. These data indicated that marrow cells with high RP59 content were recruited into growing bone tissue. RP59 may help to study the transition of bone marrow cell to osteoblast in more detail.</div></front></TEI><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Christersson, C" sort="Christersson, C" uniqKey="Christersson C" first="C" last="Christersson">C. Christersson</name><name sortKey="Kruger, A" sort="Kruger, A" uniqKey="Kruger A" first="A" last="Krüger">A. Krüger</name><name sortKey="Lundmark, C" sort="Lundmark, C" uniqKey="Lundmark C" first="C" last="Lundmark">C. Lundmark</name></noCountry><country name="Suède"><noRegion><name sortKey="Wurtz, T" sort="Wurtz, T" uniqKey="Wurtz T" first="T" last="Wurtz">T. Wurtz</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003452 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003452 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:11161722
|texte= A new protein expressed in bone marrow cells and osteoblasts with implication in osteoblast recruitment.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:11161722" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |